Plasmid

Part:BBa_K5116019

Designed by: Zhe Jing Yourong Yan   Group: iGEM24_HNU-China   (2024-10-02)


pGBKT7-CYC1-LALBA-FIG1-GalE

A plasmid that expresses UDP-glucose epimerase and lactalbumin α which forms the lactose synthesis complex with UDP- galactosyltransferase in pGADT7

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1299
    Illegal XbaI site found at 5125
    Illegal XbaI site found at 6113
    Illegal PstI site found at 1321
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1299
    Illegal PstI site found at 1321
    Illegal NotI site found at 1326
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1299
    Illegal BglII site found at 7929
    Illegal BglII site found at 9506
    Illegal BamHI site found at 1309
    Illegal XhoI site found at 979
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1299
    Illegal XbaI site found at 5125
    Illegal XbaI site found at 6113
    Illegal PstI site found at 1321
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1299
    Illegal XbaI site found at 5125
    Illegal XbaI site found at 6113
    Illegal PstI site found at 1321
    Illegal NgoMIV site found at 3099
    Illegal NgoMIV site found at 3382
    Illegal NgoMIV site found at 7132
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 119
    Illegal BsaI site found at 898
    Illegal BsaI site found at 2887
    Illegal SapI site found at 1841
    Illegal SapI site found at 3322
    Illegal SapI site found at 3532


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Categories
Parameters
None